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Cisbio to Launch GPCR Internalization Assays and More at SBS 2010
 06 Apr 2010: Cisbio Bioassays will be launching GPCR internalization assays, as well as multimode cell lines validated on all HTRF® and Tag-lite® assays (binding assays, dimer assays, second messengers, phospho-ERK and internalization). Two tutorials about Tag-lite® and GPCR pharmacology with the Tag-lite® technological platform will also be available.
Cisbio Bioassays
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New Europium Cryptates to Probe Molecular Interactions using HTRF®
12 Mar 2010: The trisbipyridine (TBP) europium cryptate currently used as energy donor in HTRF® has enabled the probing of a large number of molecular interactions. This application note shows that the europium cryptates, TBP4COOH and PBP4COOH, have interesting new photophysical properties for their use as energy donor in HTRF®.
Cisbio Bioassays
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Cisbio Bioassays Introduces Tag-lite® Multi-Mode Cell Lines
09 Mar 2010: Cisbio Bioassays introduces six multi-mode cell lines to its expanding Tag-lite® cellular platform. These new, multi-mode cell lines enable researchers to investigate the biological complexity of cell membrane receptors using the same cellular material, making Tag-lite a complete drug discovery solution for GPCR studies.
Cisbio Bioassays
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Comparison of HTRF® Indirect Systems for Mouse and Rat Monoclonal Antibody Detection
05 Mar 2010: This application note shows the uncontested superiority of specific anti-species antibodies versus Ig-binding proteins. In the series, rabbit anti-mouse polyclonal antibody always improved the detection of mouse and rat monoclonal antibodies. This was also true for the immunodetection of rabbit polyclonal antibodies.
Cisbio Bioassays
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HTRF® Check Kits for GST and 6HIS Tag Accessibility; Proof of Concept for c-myc and FLAG® Detection Systems
04 Mar 2010: Tag detection can be made difficult, in particular for small and peptidic motifs which may be hindered during protein folding. The HTRF® check kits presented here were designed as a one step procedure, with a maximum incubation time of 2 hours. The two exiting kits for GST and 6HIS checking include all necessary reagents for detection (lyophilized for better preservation) and sample preparation.
Cisbio Bioassays
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Use of 2,4-Dinitrophenyl-N-Hydroxysuccinimide Ester (DNP-NHS) for Protein Labeling and its Detection by Anti-DNP HTRF® Generic Reagents
02 Mar 2010: Anti-DNP reagents are commonly used in molecular biology for the detection of oligonucleotide probes. We have successfully optimized the labeling of 265.5 monoclonal antibody anti-DNP with europium cryptate or XL665. These HTRF® toolbox reagents have already proved their ability to detect reliably the dinitrophenyl motif in various circumstances (oligonucleotide or phosphorylated peptide detection,
caspase 3 assay...).
Cisbio Bioassays
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HTRF Assays on the New SpectraMax M5e Multi-Detection Microplate Reader
02 Mar 2010: This application note demonstrates for cAMP cell-based assays, best results were obtained using about 4000 CHO-M1 cells per well of a 384-well plate. Optimal cell density may vary according to cell type and specific assay conditions.
Cisbio Bioassays
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HTRF® IP-One Assay Performed on the PHERAstar and RUBYstar Plate Readers
01 Mar 2010: This application note shows that Cisbio’s new IP-One assay is the first high throughput system that can easily detect inositol(1)phosphate (IP1), one of the major products of the phosphatidyl inositol cascade, which tightly correlates with Gq-coupled activity.
Cisbio Bioassays
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Screening and Titration of human Fc Chimera using HTRF® Technology
28 Feb 2010: The production of human Fc tagged chimera or of humanized monoclonal antibodies has raised considerable interest in them as potential drug candidates. This application now shows the HTRF® human Fc detection assay to be fully adapted to the screening of clone libraries producing human Fc-tagged proteins.
Cisbio Bioassays
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clinical diagnostics
